Pathogens, Free Full-Text
Por um escritor misterioso
Last updated 16 junho 2024
![Pathogens, Free Full-Text](https://www.mdpi.com/pathogens/pathogens-12-00508/article_deploy/html/images/pathogens-12-00508-g001.png)
In Brazil, blood donation is regulated by the Brazilian Ministry of Health, and all States follow the same protocol for clinical and laboratory screening. Brazil is an endemic country for Chagas disease (CD), caused by Trypanosoma cruzi, and for leishmaniasis, caused by a species of Leishmania spp. Screening for leishmaniosis is not routinely performed by blood banks. Given the antigenic similarity between T. cruzi and Leishmania spp., cross-reactions in serological tests can occur, and inconclusive results for CD have been found. The objective of this study was to apply molecular techniques, e.g., nPCR, PCR, and qPCR, to clarify cases of blood donation candidates with non-negative serology for CD and to analyze the difference between the melting temperature during real-time PCR using SYBR Green. Thirty-seven cases that showed non-negative results for CD using chemiluminescent microparticle immunoassay (CMIA) tests from blood banks in Campo Grande, MS, and Campinas, SP, were analyzed. In the serum samples, 35 samples were evaluated by ELISA, and 24.3% (9/35) showed positive results for CD. nPCR was able to detect 12 positive results in 35 samples (34.28%). qPCR for T. cruzi was quantifiable in the samples that showed a value ≥0.002 par eq/mL (parasite equivalents per milliliter), and in 35 samples, 11 (31.42%) were positive. Of all evaluated samples using the described tests (CMIA, ELISA, nPCR, and qPCR), 18 (48.6%) were positive for CD. For MCA by qPCR, the melting temperature was 82.06 °C ± 0.46 for T. cruzi and 81.9 °C ± 0.24 for Leishmania infantum. The Mann–Whitney test showed a significant value of p < 0.0001. However, the differentiation between T. cruzi and L. infantum could not be considered due to temperature overlap. For leishmaniasis, of the 35 samples with non-negative serology for CD tested by the indirect fluorescent antibody test (IFAT), only one sample (2.85%) was positive (1:80). The PCR for Leishmania spp. was performed on 36 blood samples from donation candidates, and all were negative. qPCR for L. infantum showed 37 negative results for the 37 analyzed samples. The data presented here show the importance of performing two different tests in CD screening at blood banks. Molecular tests should be used for confirmation, thereby improving the blood donation system.
![Pathogens, Free Full-Text](https://www.mdpi.com/pathogens/pathogens-02-00033/article_deploy/html/images/pathogens-02-00033-g001-1024.png)
Pathogens, Free Full-Text
![Pathogens, Free Full-Text](https://zenggroup.org/wp-content/uploads/2023/09/Thermolysis_TOC.gif)
Publications – Zeng Lab
![Pathogens, Free Full-Text](https://static.primary.prod.gcms.the-infra.com/static/site/femspd/image/FEMSPD+Article+Winner+2022_480x270.png?node=60d5ca455f8a10465734&version=590054:dfc710c2423e3619f13f)
Pathogens and Disease
![Pathogens, Free Full-Text](https://www.genaxxon.com/media/image/16/c7/0f/pathogens.jpg)
Know your pathogens! Genaxxon bioscience - online shop
![Pathogens, Free Full-Text](https://study.com/cimages/videopreview/videopreview-full/important-pathogenic-bacteria4_174540.jpg)
Pathogenic Bacteria, Overview, Types & Examples - Video & Lesson Transcript
![Pathogens, Free Full-Text](https://www.pnas.org/cms/10.1073/pnas.2300970120/asset/c38785c7-ccd8-4f18-a334-7003edc67bf3/assets/images/large/pnas.2300970120fig01.jpg)
NADases as weapons for both plant pathogens and their hosts
![Pathogens, Free Full-Text](https://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13099-019-0330-9/MediaObjects/13099_2019_330_Fig2_HTML.png)
First complete genome sequence and comparative analysis of Salmonella enterica subsp. diarizonae serovar 61:k:1,5,(7) indicates host adaptation traits to sheep, Gut Pathogens
![Pathogens, Free Full-Text](https://www.cell.com/cms/asset/1c4aa418-e8e3-44d9-9275-f2ebdb34713c/gr1.jpg)
An Immunomodulatory Molecule of Symbiotic Bacteria Directs Maturation of the Host Immune System: Cell
![Pathogens, Free Full-Text](https://www.nejm.org/na101/home/literatum/publisher/mms/journals/content/nejm/2010/nejm_2010.362.issue-19/nejmra0904124/production/images/img_medium/nejmra0904124_f1.jpeg)
Hospital-Acquired Infections Due to Gram-Negative Bacteria
![Pathogens, Free Full-Text](https://www.biologyonline.com/wp-content/uploads/2019/10/pathogen-definition-and-example.jpg)
Pathogen Definition and Examples - Biology Online Dictionary
Recomendado para você
-
Relatório Focus: mercado prevê inflação de 8,89% em 2022, aponta boletim parcial16 junho 2024
-
627º Boletim Coronavírus - Morro da Fumaça16 junho 2024
-
Caso de Covid-19 é descartado em Pedro Osório16 junho 2024
-
Ler ou não ler o boletim Focus? Eis a questão - Inteligência Financeira16 junho 2024
-
Soro Específico pessoa e picada de cobra16 junho 2024
-
Pama–Nyungan grandparent systems change with grandchildren, but not cross-cousin terms or social norms, Evolutionary Human Sciences16 junho 2024
-
Batedor de Leite - Branco, Entrega Gratuita16 junho 2024
-
Landscape changes in the Campos region, southernmost Brazil, since the early deglaciation based on a multi-proxy analysis of a peat bog - ScienceDirect16 junho 2024
-
O que significa cursando no boletim escolar quer dizer que eu passei de ano?16 junho 2024
-
Os princípios constitucionais da dependência hierárquica (MF espanhol) e independência funcional (MP brasileiro): reflexões à luz da evolução do Ministério Público e do constitucionalismo contemporâneo16 junho 2024
você pode gostar
-
Bungie explains Halo: Reach ending16 junho 2024
-
Graduate Success Story: Okoko Cosmetiques16 junho 2024
-
PsBattle: Shadow the Hedgehog statute pointing a gun at Sonic the Hedgehog plushie : r/photoshopbattles16 junho 2024
-
Discord – CS Board Game Club16 junho 2024
-
ANIME* DVD DRAGON BALL Z COMPLETE TV SERIES VOL.1-291 END ENGLISH16 junho 2024
-
DBO Alternative Future16 junho 2024
-
Lego Batman E Robin em Promoção na Americanas16 junho 2024
-
Knvb Photos, Images and Pictures16 junho 2024
-
Governo de Minas anuncia programa “Wi-fi nas Escolas” para todo o estado16 junho 2024
-
Bom-Crioulo (1895) - 5 - Wattpad16 junho 2024