Expediting Antibody Discovery with a Cell and Bead Multiplexed
Por um escritor misterioso
Last updated 15 junho 2024
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://d3i71xaburhd42.cloudfront.net/6607b0810cbf8510cad934e8dcbace8076997a96/5-Figure2-1.png)
A high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health is developed that can reduce the time to antibody cloning and production. With advances in molecular engineering and humanization, monoclonal antibodies are one of the fastest-growing classes of biopharmaceuticals. During antibody discovery, antibody from hybridoma or primary B-cell supernatants is screened for the desired binding characteristics, and secondary screens measure antibody function and concentration, identify immunoglobulin G (IgG) isotype, and assess cell health. In order to expedite the antibody discovery process, we developed a high-throughput, multiplexed cell and bead-based competition assay that identifies and quantitates mouse IgG isotypes and assesses cell health. No differences in assay performance were observed between single and multiplex formats. The linear range of the assay was from 0.5 to 50 µg/mL, and washing was not required, decreasing assay time and variability. Slight modifications to the protocol allowed quantification of dilute antibody supernatants (0.1–5 µg/mL). Using hybridoma cultures, we showed that cell viability measurements in the assay did not interfere with the bead-based IgG measurements. The assay described here is a simple mix-and-read, no-dilution screen that can reduce the time to antibody cloning and production. The high-content data can differentiate monoclonal and polyclonal wells, determine IgG quantity for downstream functional assays, provide isotype information, and monitor cell proliferation and viability.
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://pages.nxtbook.com/nxtbooks/gen/intellicyt/iphone/gen_intellicyt_p0006_midres.jpg?1616159644)
Antibody Screening Solutions Made Easy - 6
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://www.slas.org/SLAS/assets/Image/eln/072318_eln-article-banner.jpg)
High-Powered Multiplexing with Flow Cytometry in High-Throughput
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://i.ytimg.com/vi/EWVjoS2rH74/hqdefault.jpg)
Uploads from Cell Signaling Technology, Inc.
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://media.springernature.com/lw685/springer-static/image/chp%3A10.1007%2F978-1-0716-2978-9_26/MediaObjects/522477_3_En_26_Fig1_HTML.png)
Accessing Antibody Reactivities in Serum or Plasma to (Auto
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://d2jx2rerrg6sh3.cloudfront.net/image-handler/picture/2022/3/10188_a1-2.jpg)
Using bead-based, high throughput panels to improve the quality of
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://cdn.10xgenomics.com/image/upload/v1683243616/marketo-pdfs/BEAM-Ab_AAI_2023.png)
High-throughput antibody discovery using Barcode Enabled Antigen
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://journals.sagepub.com/cms/10.1177/2472555218776308/asset/images/large/10.1177_2472555218776308-fig4.jpeg)
Expediting Antibody Discovery with a Cell and Bead Multiplexed
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://resources.rndsystems.com/images/datasheets/lxsapm_porcine-luminex-discovery-assay-9620211173.png)
Porcine Luminex® Discovery Assay (LXSAPM): R&D Systems
![Expediting Antibody Discovery with a Cell and Bead Multiplexed](https://ars.els-cdn.com/content/image/1-s2.0-S2472555222068939-gr1.jpg)
Expediting Antibody Discovery with a Cell and Bead Multiplexed
Recomendado para você
-
Omega Legends - Apps on Google Play15 junho 2024
-
Shotgun King IGG Games The Final Checkmate v1.39 Download15 junho 2024
-
Microarray assessment of N-glycan-specific IgE and IgG profiles15 junho 2024
-
Biology, Free Full-Text15 junho 2024
-
Altered Fc galactosylation in IgG4 is a potential serum marker for15 junho 2024
-
Widespread vertical transmission of secretory immunoglobulin A15 junho 2024
-
TheNightcapKing - Hobbyist, General Artist15 junho 2024
-
Optimization of an Experimental Vaccine To Prevent Escherichia15 junho 2024
-
Brainworx bx_opto - Electronic Delivery - Vintage King15 junho 2024
-
Bigg TV15 junho 2024
você pode gostar
-
Why do Real Madrid players smell the chests of their jerseys during15 junho 2024
-
Z-O-M-B-I-E-S 2 sdílel(a) fotku na Instagramu: „#zombies215 junho 2024
-
BEMAX - YAMETE KUDASAI!15 junho 2024
-
Juegos Panamericanos Santiago 2023: ¿Dónde y cómo comprar entradas para el evento en Chile? — LOS40 Chile15 junho 2024
-
Fan-made Fatality animation for Mortal Kombat 2 receives praise from Ed Boon himself15 junho 2024
-
Imminent Escape? - RJ Writing Ink15 junho 2024
-
List of Katekyo Hitman Reborn! chapters and volumes Hitman reborn, Reborn katekyo hitman, Reborn manga15 junho 2024
-
NUEVO HACK De Subway Surfers 2022✓- Todos los personajes y TODO15 junho 2024
-
Shirakami Fubuki Ace Combat 7 (Final Mission) by 73RO on DeviantArt15 junho 2024
-
TIER LIST LEGENDARIOS ACTUALIZADA 3 - ANIME MANIA ROBLOX15 junho 2024